The offered position is joint between the labs of Verena Ruprecht and Manuel Irimia. The Ruprecht lab studies cell and tissue dynamics during early vertebrate development by advanced molecular and biophysical techniques and quantitative high-resolution live cell imaging approaches. The lab follows an interdisciplinary approach based on in-vivo studies of Zebrafish development, complemented by primary progenitor cell cultures to study complex three-dimensional tissue rearrangements and patterning in the embryo. The Irimia lab combines high throughput transcriptomics with in vivo experiments to elucidate the roles of alternative exons in vertebrate development and evolution. In particular, they are trying to characterize the roles of neuronal-specific microexons in brain development and mature function using the CRISPR-Cas9 system and wide variety of tests.
We seek a highly motivated and organized researcher to assist on a wide diversity of research projects involving zebrafish development. Tasks will include (depending on the specific project needs): colony management and fish husbandry, transgenesis by zygotic injection, embryology techniques (in situ hybridization and immunohistochemistry, cell transplantation, etc.), and basic molecular biology (genotyping, PCRs, in vitro transcription, cloning, etc.).
The research technician is expected to take a pro-active role in supporting ongoing research projects and taking responsibility in zebrafish colony management.